1) Turn on the PCASP pump by connecting the black control box to the instrument and flip the switch to the On position. Use a flow standard, such as the Gilibrator. Use the smallest Gilian container and connect tube to the bottom hole (see picture below).
2) Measure the concentration at the exit of the flow system (see picture below)
3) Use the black button by the tube to take 6 samples with the Gilibrator and the average flow rate should be around 60 ccm.
Note that the calibration is for one refractive index and size. Typically, there will be particles from other sizes due to not having pure (ultra clean works best) water or reusing the nebulizers. The best method to clean the nebulizers is to rise them with pure (distilled) water many, many times. Helps to store the nebulizers in distilled water and seal the nebulizers.